POWER-Kits™for Freund’s Adjuvant
Perfect Emulsions Every Time Affordable for Everybody
Revolutionary Technology
Our unique technology (patent pending) has revolutionized the process of making Freund’s Adjuvant emulsions (incomplete or complete) containing the antigen of your choice. It also works with Montanide™ and other oil adjuvants to prepare water-in-oil emulsions.
With the MedTech device POWER (Perfect Oil in Water Emulsion Receptacle) and a homogenizer, it is possible to introduce a global standardization process. This will ensure high-quality emulsions for research and the veterinary sector.
Malin Hultqvist, CEO, Redoxis
Reproducible high-quality emulsions are essential for generating reliable and consistent results.
Malin Hultqvist, CEO, Redoxis
More advantages with
POWER-Kit™
Flexible
Prepare 2-8 ml of emulsion with your adjuvant and antigen of choice.
Affordable
Compared with the 2-syringe method, break-even after 310 immunisations.
Efficient
Prepare 3 different emulsions in 10 minutes.
Simple
Easy to learn and little “hands-on” time.
Safe
No risk of accidental exposure or Repetitive Stress Injuries (RSI).
Minimal Waste
No more doubling of the amount needed, as with the 2-syringe method.
Produce Perfect Emulsions
Here we describe how to produce a perfect emulsion, using POWER-Kits™ and Bertin’s MiniLys Homogenizer. One can produce 3 different emulsions in about 10 minutes. Watch the video or read how the emulsions should be prepared.
Follow these steps on how to prepare perfect emulsions.
- Put the receptacle and all necessary reagents to be used in ice
- Add PBS, antigen and other aqueous based solutions to the receptacle
- Lastly add the adjuvant (Freund’s, Montanide , etc.)
- Tighten the lid firmly
- Shake the receptacle for 5 seconds by hand
- Place it in the MiniLys Homogenizer and secure it with the rod
- Set time to 60 seconds and choose the highest speed
- Push start
- When complete, place the receptacle on ice and cover it completely
- After 3 minutes, repeat shaking for another 60 seconds on the highest setting
- Centrifuge the receptacle for 1 minute at 300g to recover all the emulsion
- Remove the lid and insert the plunger
- Push it down so that it just touches the top of the emulsion
- Tear off the twist-off piece at the bottom of the receptacle
- Remove the green plunger (if using Braun’s Injekt®-F Solo syringes) from the 1ml syringe and save for later use
- Lock in the back end of the syringe barrel to the receptacle with a short twist
- Push the plunger into the receptacle until the emulsion reaches the 0,15ml mark on the syringe-barrel
- Remove the syringe barrel from the receptacle and attach a needle (we recommend a 25G x 16mm (⅝”) hypodermic needle for mice)
- Take the green plunger and slowly insert it into the syringe barrel, making sure no air is trapped
- Push the plunger in further until the emulsion comes out of the needle
- Continue until all the syringes are filled with the emulsion
Direct Cost for the EAE model
Comparing the cost of the POWER-Kit™ method with the 2-syringe method after 800 immunisations. In this example, costs associated with research for human Multiple Sclerosis, using the EAE model have been calculated.
Break-even point
about 310 immunisations
* Up to 800 immunisations.
€ 349 for Profit organisations
** Calculated with a 50% loss of emulsion, more with smaller volumes.
POWER-Kit™Method
SHAKING MACHINE€ 2,990
POWER KIT€ 299*
REAGENTSAbout € 20/immunisation
800 immunisations
€ 19,289
Saves € 4,721
2-SyringeMethod
-
SYRINGE, STOPCOCK etcabout € 10
REAGENTSAbout € 30/immunisation**
800 immunisations