POWER-Kit™ can be used for:
- Preparation of emulsions with Freund´s Adjuvant + Antigens
- Safe and homogeneous preparation of M.tuberculosis suspensions
- Monoclonal & polyclonal antibody production
- DHT experiments
-
Preclinical animal models for research & in industry
- Rheumatoid Arthritis (CIA)
- Multiple Sclerosis (EAE)
- Autoimmune Uveitis (EAU)
- Mysasthenia Gravis (MG)
- Cancer Immunotherapy
- etc …
Preparing Perfect Emulsions
Every Time and Affordable for Everybody
Shaken, not stirred
POWER-Kit™ test results
Consistent Quality Emulsions
Yield consistent results with POWER-Kit™
1
Induction of disease in the animal model for human Multiple Sclerosis, EAE
Emulsions were prepared fresh each time, using POWER-Kits™. Five different experiments were set up, at least 1 month apart. Mice, C57BL/6J, were bought from Janvier and were delivered 1 week before each experiment (5 in each group). Experiments were performed by staff from BTB Emulsions.
2
Induction of disease in the animal model for human Rheumatoid Arthritis, CIA
Emulsions were prepared using POWER-Kit™. DBA/1 mice (10 in each group) were immunized on day 0 and boosted on day 28, then observed for disease induction. The experiment was performed by an independent CRO (Redoxis AB, Lund, Sweden).
Interchangeable Method
Indistinguishable results compared with the 2-syringe method
3
Induction of disease in the animal model for human Multiple Sclerosis, EAE
Emulsions were prepared using POWER-Kit™ or the traditional 2-syringe method. C57BL/6J mice (10 in each group) were immunized day 0 and observed for disease induction. The experiment was performed by an independent CRO (Redoxis AB, Lund, Sweden).
Top Performance Every Time
Yield homogenous and stable emulsions
4
Top performance with homogenous emulsions with POWER-Kit™
Emulsions prepared using POWER-Kit™, traditional 2-syringe method, or Vortex. A small amount of emulsion was placed and a cover-slip and observed under a Bright-Field microscope (400x) and photos captured with a digital camera. Emulsions prepared with Vortex, do NOT induce EAE (results not shown).
5
Top performance with stable emulsions with POWER-Kit™
One emulsion was prepared using POWER-Kit™. The emulsion was stored at +4 degrees Celsius and particle size measured at the indicated number of weeks after preparation. The experiment was performed by Hans Bolinsson, Lund University, Faculty of Engineering, using a Malvern Mastersizer 2000.
Quality Control
The CEO of BTB Emulsions, Thomas Bäckström, has years of experience working with pre-clinical animal models and emulsions. “Early in my career, I figured out that the ‘water-drop test’ did not predict whether or not the emulsion induced disease in the human model for Multiple Sclerosis, EAE.” With POWER-Kit™ and a shaking homogenizer one can produce optimal emulsions every time. The quality of the emulsion is best determined by using a Bright-Field microscope and/or a particle size analyzer (see figures 4 & 5 above).
Comparing Methods
Test results, using pre-clinical models, show many advantages using the new technology POWER-Kit™.
POWER-Kit™
2-Syringe
Performance
Performance
Superior emulsions independent of the operator
Performance
Huge person-person variation
Affordability
Performance
Initial investment, then start saving after 310 immunisations
Performance
No initial investment
Flexibility
Performance
Compatible with Freund’s and other oil adjuvants, and any antigen
Performance
Freund’s and other oil adjuvants react with the rubber on 3-part syringes
Quality
Performance
Perfect quality every time
Performance
Huge variation in the quality of emulsion
Waste
Performance
No waste of expensive materials
Performance
Huge waste of expensive materials
Time
Performance
Three different emulsions in 10 minutes*
Performance
One emulsion in 15-20 minutes
* With PrecelLys Evolution, prepare up to 24 different emulsions in 10-15 minutes
How to Prepare Perfect Emulsions
Every Time and Affordable for Everybody
Follow these steps how to prepare perfect emulsions.
- Put the receptacle and all necessary reagents to be used in ice
- Add PBS, antigen and other aqueous based solutions to the receptacle
- Lastly add the adjuvant (Freund’s, Montanide etc.)
- Tighten the lid firmly
- Shake the receptacle for 5 seconds by hand
- Place it in the MiniLys Homogenizer and secure it with the rod
- Set time to 60 seconds and choose the highest speed
- Push start
- When complete, place the receptacle on ice and cover it completely
- After 3 minutes, repeat shaking for another 60 seconds on the highest setting
- Centrifuge the receptacle for 1 minute at 300g to recover all the emulsion
- Remove the lid and insert the plunger
- Push it down so that it just touches the top of the emulsion
- Tear off the twist-off piece at the bottom of the receptacle
- Remove the green plunger (if using Braun’s Injekt®-F Solo syringes) from the 1ml syringe and save for later use
- Lock in the back end of the syringe-barrel to the receptacle with a short twist
- Push the plunger into the receptacle until the emulsion reaches the 0,15ml mark on the syringe-barrel
- Remove the syringe-barrel from the receptacle and attach a needle (we recommend a 25G x 16mm (⅝”) hypodermic needle for mice)
- Take the green plunger and slowly insert it into the syringe-barrel, making sure no air is trapped
- Push the plunger in further until the emulsion comes out of the needle
- Continue until all the syringes are filled with the emulsion